The United States District Court for the Eastern District of New York (in case citations, E.D.N.Y.) is the federal district court whose jurisdiction comprises Long Island (including portions of New York City) and Staten Island in New York. The court's territorial jurisdiction includes the counties of Kings (Brooklyn), Queens, Richmond (Staten Island), Nassau, and Suffolk as well as, concurrently with the Southern District of New York, the waters of New York and Bronx counties (including New York Harbor and the East River). Courthouses are located in Brooklyn and Central Islip.

The United States Attorney's Office for the Eastern District of New York represents the United States in civil and criminal litigation in the court. The Acting United States Attorney for the Eastern District of New York since June 2021 is Jacquelyn M. Kasulis.

Appeals from the Eastern District of New York are taken to the United States Court of Appeals for the Second Circuit (except for patent claims and claims against the U.S. government under the Tucker Act, which are appealed to the Federal Circuit).